DNA typing from skeletal remains using GlobalFiler™ PCR amplification and Investigator® 24plex QS kits
نویسندگان
چکیده
منابع مشابه
DNA typing from skeletal remains: evaluation of multiplex and megaplex STR systems on DNA isolated from bone and teeth samples.
AIM To evaluate the performance of three multiplex short tandem repeat (STR) systems (AmpflSTR Profiler, AmpflSTR Profiler Plus, and AmpflSTR COfiler), and a megaplex STR system (PowerPlex 16) on DNA extracted from the skeletal remains. By performing a microbial DNA challenge study, we also evaluated the influence of microbial DNA on human DNA typing. METHODS A subset of 86 DNA extracts isola...
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The survival of DNA, the most informative biological molecule, for periods of at least several thousand years in bone was demonstrated more than four years ago. However, difficulties with authenticating ancient DNA have made diagenetic studies problematic. It is therefore essential that these problems be overcome before the question of DNA survival can be addressed. Here we describe our work wi...
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AIM To perform an efficiency study of three new amplification kits with the extended European Standard Set (ESS) of loci for autosomal short tandem repeat (STR) typing of skeletal remains excavated from the World War II mass graves in Slovenia. METHODS In the beginning of the 2011, we analyzed 102 bones and teeth using the PowerPlex ESX 17 System (Promega), AmpFiSTR NGM PCR Amplification Kit ...
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AIM To comparatively test nine co mmercially available short tandem repeat (STR)-multiplex kits (PowerPlex 16, 16HS, ES, ESI17, ESX17, S5 [all Promega]; AmpFiSTR Identifiler, NGM and SEfiler [all Applied Biosystems]) for their efficiency and applicability to analyze ancient and thus highly degraded DNA samples. METHODS Fifteen human skeletal remains from the late medieval age were obtained an...
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BACKGROUND Identification of pathogen DNA from archaeological human remains is a powerful tool in demonstrating that the infectious disease existed in the past. However, it is very difficult to detect trace amounts of DNA remnants attached to the human skeleton, especially from those buried in a humid atmosphere with a relatively high environmental temperature such as in Asia. METHODOLOGY/PRI...
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ژورنال
عنوان ژورنال: Forensic Science International: Genetics Supplement Series
سال: 2019
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2019.09.021